PRRSV重组N蛋白的抗原性分析及其特异性抗体制备

利用大肠杆菌表达技术和亲和层析法纯化出重组PRRSV N蛋白 ,用间接ELISA法和Western blot分析 ,证明其具有较好的抗原性。将该纯化蛋白采用大剂量长程免疫法免疫家兔 ,制备了兔抗重组N蛋白抗体 ,用Western blot分析和竞争抑制试验证明其具有很高的特异性 ,ELISA效价达 1∶32 0 0以上。     

p5CS基因在蒙农杂种冰草植株中的表达及耐盐性研究

为尽快培育出适宜我国干旱荒漠地区大面积推广的耐盐冰草新品种,试验以经PCR和Southern blot杂交检测的含有p5CS基因的蒙农杂种冰草植株为材料,用Northern blot检测目的基因在转化植株中的表达;并用1.5%NaCl盐溶液进行胁迫处理确定转化植株的耐盐性。结果表明:转基因冰草植株与DIG标记探针杂交呈现明显的杂交带;盐胁迫下,游离脯氨酸含量增加较快,质膜透性、丙二醛含量增加较小,SOD活性较高。说明p5CS基因能够在冰草基因组的转录水平上表达,表达植株的耐盐性明显增加。     

中国西部典型区生态环境本底遥感调查

1997年我国实施了西部大开发战略,西部地区的生态环境问题再次引起多方面的关注。“西部金睛行动计划”的实施旨在通过具有代表意义的7个典型区同步监测西部10年(1990～2000年)来的生态环境变化情况,为国家西部大开发战略提供科学的决策支持。项目采用遥感与地理信息系统技术相结合的方法,按照8个统一的原则,制订了生态环境遥感监测技术指南,对这些地区在1990年和2000年的生态环境变化进行了动态监测。监测结果表明,这10年来,由于人类活动加强,农业用地、城市用地大量增加,挤占了自然/半自然植被区,草场过牧引起草场退化,致使典型区生态环境仍处于逐渐恶化的状态。     

Field compaction at different soil-water status: effects on pore size distribution and soil water characteristics of a Rhodic Ferralsol in Western Cuba

The level of compaction induced on cultivated fields through trafficking is strongly influenced by the prevailing soil-water status and, depending on the attendant soil degradation, vital soil hydraulic processes could be affected. Therefore, understanding the relationship between field soil-water status and the corresponding level of induced compaction for a given load is considered an imperative step toward a better control of the occurrence of traffic-induced field soil compaction. Pore size distribution, a fundamental and highly degradable soil property, was measured in a Rhodic Ferralsol, the most productive and extensively distributed soil in Western Cuba, to study the effects of three levels of soil compaction on soil water characteristic parameters. Soil bulk density and cone penetration index were used to measure compaction levels established by seven passes of a 10 Mg tractor at three soil-water statuses corresponding to the plastic (Fs), friable (Fc) and relatively dry soil (Ds) consistency states. Pore size distribution calculated from soil water characteristic curves was classified into three pore size categories on the basis of their hydraulic functioning: >50 μm (f_>50 μm), 50–0.5 μm (f_{50–0.5 μm}) and <0.5 μm (f_<0.5 μm). The greatest compaction levels were attained in the Fs and Fc soil water treatments, and a significant contribution to compaction was attributed to the existing soil water states under which the soil compaction was accomplished. Average cone index (CI) values in the range of 2.93–3.70 MPa reflected the accumulation of f_<0.5 μm pores, and incurred severe reductions in the volume of f_>50 μm pores in the Fs and Fc treatments, while an average CI value of 1.69 MPa indicated increments in the volume of f_{50–0.5 μm} in the Ds treatment. Despite the differential effects of soil compaction on the distribution of the different pore size categories, soil total porosity (f_Total) was not effective in reflecting treatment effects. Soil water desorption at the soil water potentials evaluated (0.0 to −15,000 cm H₂O) was adversely affected in the f_<0.5 μm dominated treatments; strong soil water retention was observed with the predominance of f_<0.5 μm, as was confirmed by the high water content at plant wilting point. Based on these findings, the use of field capacity water content as the upper limit of plant available soil water was therefore considered inappropriate for compacted soils.     

投影寻踪技术在松嫩平原西部水资源可持续利用中的应用

根据松嫩平原西部水资源复合系统的特征和水资源条件,建立了区域水资源可持续利用评价指标体系。应用投影寻踪技术,采用实码遗传算法优化投影指标函数,将方案多维评价指标值综合为一维投影值,根据投影值的大小实现方案的优选。研究表明,投影寻踪技术可以有效地解决权重的人为干扰,通过优化排序实现对评价结果的定量化表征。     

Management strategies to increase stand structural diversity and enhance biodiversity in coastal rainforests of Alaska

Coastal rainforests of southeast Alaska have relatively simple species composition but complex structures with high diversity of tree ages, sizes and forest canopy layers, and an abundant understory plant community. Wildlife and fisheries resources also play an important role in the ecological functioning of forest and aquatic systems. Clearcutting has greatly altered these forest ecosystems with significant decreases in structural diversity of forest stands and greatly reduced wildlife habitat. This paper synthesizes information on management options in older forests that have never been actively managed, and in younger forests to increase diversity of stand structures and their associated effects on biodiversity. Light to moderate levels of partial cutting in old-growth forests can maintain the original diversity of overstory stand structures and understory plant communities. In younger forests that develop after clearcutting, mixed alder-conifer stands provide more heterogeneous structures and significantly higher understory biomass than in pure conifer forests. Research has shown that red alder increases diversity and abundance of understory plants, and provides forage for deer and small mammals. Results also show a clear linkage between alder and improved invertebrate diversity in aquatic systems. A combination of light partial cutting in older forests along with inclusion of red alder in conifer-dominated forests could provide the greatest amount of diversity and maintain the complex stand structures that are an important component of these forest ecosystems.     

Composition,distribution and sources of polycyclic aromatic hydrocarbons in sediments of the western harbour of alexandria,egypt

Aim and Background  Little data are available on the levels of polycyclic aromatic hydrocarbons (PAHs) in the sediments of the Mediterranean Sea Coast of Egypt in general and the Alexandria coastal zone in particular. It was therefore deemed necessary to set up a monitoring programme to determine the current concentrations of PAHs in bottom sediments, and to identify any area where high concentrations of these potential hazardous contaminants were present in the Western Harbour of Alexandria. Methods  The composition, distribution and the source of PAHs in surficial sediments of the harbour were investigated. To document the spatial PAH input, surficial sediment samples from 23 locations throughout the harbour were analysed. as]Results and Discussion The total PAH load determined in the surficial sediment samples ranged from 8 to 131150 ng g¹ dry wt, generally with most of the samples having total concentrations of PAHs greater than 5000 ng g¹ dry weight. The highest concentration of total PAHs was recorded in sediments of the inner harbour. Ratio values of specific compounds such as phenanthrene to anthracene, fluoranthrene to pyrene, methyl-phen-anthrene to phenanthrene, methyl-dibenzothiophenes to dibenzothiophenes, alkylated to non-alkylated and high molecular weight to low molecular weight PAH, were calculated to evaluate the possible source of PAH contamination in the harbour sediments. Conclusions  Two main sources of PAH in the study area have been found: pyrolytic and petrogenic. Interferences of rather petrogenic and pyrolytic PAH contaminations were noticed for most samples. The dominant sources of PAH appear to be the combustion processes through run-off, industrial and sewage discharges, and atmospheric input. The concentrations of PAHs were generally above levels expected to cause adverse biological effects. Recommendations and outlook  Information from this study and any other relevant studies should be useful in designing future strategies for environmental protection and management of the harbour.     

防控有害入侵生物——西花蓟马

西花蓟马(Frankliniella occidentalis)是一种世界著名的危险性害虫,我国大陆于2003年首次报道。西花蓟马的发生给农作物造成严重危害和经济损失。本文就西花蓟马的寄主与危害及其防控等方面进行了概述。     

豫西地区不同类型农田化肥增产效益及增产潜力研究

 【目的】探讨化肥在豫西地区不同地形农田的增产效益与增产潜力，为引导和实现化肥的优化配置和合理施用、提高豫西地区粮食产量提供参考依据。【方法】采用田间试验，研究了豫西地区平川地、坡平地、坡地等3种典型地形农田化肥施用量对作物产量的影响；并对3种典型农田化肥消费量与粮食产量的多年统计资料进行了分析，提出了二者之间的关系，并由此对豫西地区粮食增产潜力进行了预测。【结果】作物单产以平川地最高，坡平地次之，坡地最低。尽管在3种类型农田上使用化肥均可大幅度增加作物产量，但化肥增产效益以坡地最高，坡平地次之，平川地最低；对3种典型地形农田化肥消费量与粮食产量多年统计资料的分析也获得相同结论。根据田间试验结果对未来豫西地区粮食产量及增产潜力进行的预测表明，当采用本研究中最大施肥量时，豫西地区粮食总产量可达到4.24×109 kg，是2003年该地区粮食产量的2.1倍；并预测在增加粮食产量中，坡地贡献率占50.1%、坡平地占33.7%、平川地占16.2%，坡地和坡平地粮食生产对该地区粮食总产量具有决定作用。【结论】豫西地区3种典型地形农田中化肥增产效益存在差异，以坡地化肥增产效益最大。因此，加强坡地化肥投入，可有效地提高本区粮食总产量，对今后本区粮食的增产具有重要意义。     

豫西地区石粉矿物元素含量分析

对豫西地区石粉中矿物元素的含量进行检测,结果表明,石粉中含有丰富的钙和铁等元素。其中龙门山石粉中钙的含量为18.00%,铁含量为4 357.36 mg/kg;新安县石粉中钙的含量为32.77%,铁含量为5 398.06 mg/kg。2种石粉是补充禽用饲料中钙、铁等元素的良好来源。